ArticlesScientific Apparatus and Laboratory Methods

SEDIMENTATION OF POLIOMYELITIS VIRUS BY CENTRIFUGATION

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Science  12 Oct 1945:
Vol. 102, Issue 2650, pp. 380-382
DOI: 10.1126/science.102.2650.380

Abstract

A technic is described whereby poliomyelitis virus may be sedimented by centrifugation for four hours at 18,000 r.p.m.

Virus has been recovered quantitatively in the sediment from a 20 per cent., but not a 1 per cent., saline suspension. The addition of 10 per cent. normal serum results in quantitative recovery from a 1 per cent. suspension.

Virus has been recovered by intracerebral inoculation from two of four human stools tested by the method described.

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