Measurement of Precipitin Reactions in the Millimicrogram Protein-Nitrogen Range

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Science  26 Dec 1958:
Vol. 128, Issue 3339, pp. 1625-1626
DOI: 10.1126/science.128.3339.1625


Exploitation of newer instrumentation and a dye-binding method for protein measurement made possible reduction in volume down to 1/1000 that commonly used, with no greater error. The procedure was tested at two levels, 1- and 10-µl volumes, with human gamma globulin and rabbit antiserum. Of the dyes tested, bromsulfalein proved best for the protein estimation.