Purification of Folic Acid

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Science  30 Jan 1959:
Vol. 129, Issue 3344, pp. 274-275
DOI: 10.1126/science.129.3344.274


Various purification procedures for folic acid were investigated. The criteria of purity were a negative Bratton-Marshall test and the absence of fluorescent spots on paper chromatograms. Since no method provided a pure product, a procedure consisting of cellulose column chromatography followed by filtration through charcoal was developed.