Efficient Method for Selection of Auxotrophic Mutants of Neurospora

See allHide authors and affiliations

Science  27 Feb 1959:
Vol. 129, Issue 3348, pp. 572
DOI: 10.1126/science.129.3348.572


A technique for the selection of induced and spontaneous auxotrophic mutants of Neurospora in relatively high frequency is described. The method takes advantage of the reduced rate of death of germinating conidia of an inositol-requiring strain when a secondary mutation is imposed.