Color Reaction for Certain Amino Acids, Amines, and Proteins

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Science  12 Jun 1959:
Vol. 129, Issue 3363, pp. 1617-1618
DOI: 10.1126/science.129.3363.1617


Proteins, certain amino acids, and amines undergo a potentially useful color reaction. The reaction involves the apparent formation of pyrroles when the compounds are allowed to react with acetonylacetone. The pyrroles yield colored complexes on coupling with p-dimethylaminobenzaldehyde. This report describes the specificity and possible uses of this reaction in colorimetric measurements and in paper chromatographic detection of these compounds.