Effect of Diisopropylfluorophosphate on Sulfhydryl Proteases

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Science  19 Jun 1959:
Vol. 129, Issue 3364, pp. 1678
DOI: 10.1126/science.129.3364.1678


Diisopropylfluorophosphate inhibited all the sulfhydryl proteases studied in our tests. This inhibition was most pronounced at pH 6.0. By first blocking the sulfhydryl group with p-chloromercuribenzoate, inhibition could be prevented. Neither cysteine nor choline gave appreciable reactivation of diisopropylfluorophosphate-inhibited bromelain.