Use of Phosphorus-32 in Microassay for Nucleic Acid Synthesis in Escherichia coli

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Science  14 Aug 1959:
Vol. 130, Issue 3372, pp. 386-387
DOI: 10.1126/science.130.3372.386


A method was developed to facilitate studies of ribonucleic acid and deoxyribonucleic acid synthesis in growing bacterial cultures of small volume. The nucleic acid fractions were separated by means of a modified Schmidt-Thannhauser procedure applied to micro quantities of P32-labeled cells collected on membrane filters. The validity and usefulness of the method are discussed.