Alpha-Keto Acids in Vitamin-Free Casein Hydrolyzates (Acid)

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Science  28 Aug 1959:
Vol. 130, Issue 3374, pp. 505-506
DOI: 10.1126/science.130.3374.505


α-Ketoglutaric and pyruvic acids were isolated as their 2-4-dinitrophenylhydrazones from five different commercial samples of vitamin-free acid hydrolyzates of casein. In addition, one sample yielded traces of α-ketobutyric acid. The hydrazones were converted by hydrogenation to glutamic acid, alanine, and α-aminobutyric acid and identified by paper chromatography.