Mechanism of Enzymatic Oxidation of Purines

See allHide authors and affiliations

Science  18 Sep 1959:
Vol. 130, Issue 3377, pp. 711-712
DOI: 10.1126/science.130.3377.711-a


Tautomeric forms of 2-hydroxypurine, in which the structure has been fixed by introduction of an N-methyl group, are oxidized differently by xanthine oxidase. The 1-methyl derivative is attacked at position 8 and the 3-methyl derivative at carbon atom 6. These observations indicate that 2-hydroxypurine itself reacts in a tautomeric form, corresponding to the structure of its 1-methyl derivative.