Serological Procedure for the Detection of Antibodies to Penicillin

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Science  27 Nov 1959:
Vol. 130, Issue 3387, pp. 1472
DOI: 10.1126/science.130.3387.1472


A hemagglutination technique for demonstrating antibodies to penicillin in sera from penicillin-allergic subjects is described. Erythrocytes coupled to penicillin T by means of bis-diazotized-benzidine constitute the "antigen" used to measure the antipenicillin antibody. The hemagglutination reaction is highly specific. as indicated by hemagglutination-inhibition studies. The clinical significance of these antibodies remains to be elucidated.