Glucuronidase Activation: Enzyme Action at an Interface

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Science  08 Jan 1960:
Vol. 131, Issue 3393, pp. 101-102
DOI: 10.1126/science.131.3393.101-a


The potentiating action of chloroform on bacterial β-glucuronidase has been shown to increase as the interface area between the two liquid phases increases. Prior extraction of the enzyme with chloroform causes a loss rather than an increase in activity. It is tentatively suggested that the correlation between activity and interface area may reflect a phenomenon of enzyme action at a liquid/liquid interface.