Separation of Serum Antibody Activities by Anion-Exchange Cellulose Chromatography

Science  19 Feb 1960:
Vol. 131, Issue 3399, pp. 500-501
DOI: 10.1126/science.131.3399.500


Mumps, Histoplasma capsulatum. thyroglobulin, and typhoid H antibodies were found in fraction 1, which contained only gamma globulins with ultracentrifugal sedimentation coefficients of 6.6 Svedberg units (S). Typhoid O antibodies and rheumatoid factor were only in fraction 5, composed principally of gamma macroglobulins with ultracentrifugal sedimentation coefficients of 18 S. In contrast isohemagglutinins, Rh antibodies, and anti-human liver nucleoprotein activities were found in two chromatogram fractions (1 and 5) with different physicochemical properties.