Synchronization of Division in Escherichia coli

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Science  15 Apr 1960:
Vol. 131, Issue 3407, pp. 1098
DOI: 10.1126/science.131.3407.1098


The smallest cells of a culture in the logarithmic phase, when isolated by a single rapid filtration through 1.2-/u Millipore paper, show good synchronization and reproducibility through the first division cycle. The method minimizes metabolic shock and provides a culture in which an easily reproducible initial state is established at a known time.