New Method for Manipulation, Maintenance, and Cloning of Single Mammalian Cells in vitro

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Science  06 May 1960:
Vol. 131, Issue 3410, pp. 1376-1377
DOI: 10.1126/science.131.3410.1376


Individual mammalian cells can be isolated with the aid of easily handled glass beads to which the cells have become attached. Procedures for the preparation of cell cultures on beads, and for the recognition and manipulation of beads carrying single monocytes, HeLa cells, and Detroit-98 cells are described. Data obtained with Brucella infected monocytes, illustrating the efficiency of the method, are presented.