Reversible Inhibition ofn Beef Heart Cytochrome c Oxidase by Polyionic Macromolecules

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Science  01 Jul 1960:
Vol. 132, Issue 3418, pp. 43-44
DOI: 10.1126/science.132.3418.43


The basic proteins protamine (sulfate), histone, lysozyme, and ribonuclease were found to be potent inhibitors of mammalian heart muscle cytochromec oxidase. Their inhibitions were completely reversed in the presence of a strongly anionic polyglucose sulfate. With fresh rat heart muscle homogenates, Keili and Hartree type of beef heart muscle particulates, and deoxycholate-solubilized oxidase preparations, the reversible nature of the phenomenon was demonstrated with manometric and spectrophotometric assays for cytochrome c oxidase.