Articles

Reversible Inhibition ofn Beef Heart Cytochrome c Oxidase by Polyionic Macromolecules

+ See all authors and affiliations

Science  01 Jul 1960:
Vol. 132, Issue 3418, pp. 43-44
DOI: 10.1126/science.132.3418.43

Abstract

The basic proteins protamine (sulfate), histone, lysozyme, and ribonuclease were found to be potent inhibitors of mammalian heart muscle cytochromec oxidase. Their inhibitions were completely reversed in the presence of a strongly anionic polyglucose sulfate. With fresh rat heart muscle homogenates, Keili and Hartree type of beef heart muscle particulates, and deoxycholate-solubilized oxidase preparations, the reversible nature of the phenomenon was demonstrated with manometric and spectrophotometric assays for cytochrome c oxidase.

Related Content