Isolation of Antibodies to Gelatin from Antigen-Antibody Complex by Proteolysis

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Science  08 Jul 1960:
Vol. 132, Issue 3419, pp. 86-87
DOI: 10.1126/science.132.3419.86


Antibodies were isolated by digestion of the gelatin-antigelatin complex with collagenase and removal of the gelatin fragments by dialysis. The pure antibody could be precipitated anew by the antigen. Antibodies to tyrosylgelatin were isolated in a similar way, but in this case peptides containing the active antigenic sites remained bound to the antibody.