Preparation of Monolayer Cell Cultures from Tissues of Some Lower Vertebrates

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Science  23 Dec 1960:
Vol. 132, Issue 3443, pp. 1890-1891
DOI: 10.1126/science.132.3443.1890


Cold trypsin dispersion at pH 7.2 was used to obtain cultivable cells and cell groups from tissues of six species of fresh-water bony fishes, a frog, and a turtle. The cells readily attached to glass and were capable of at least limfted, and in some cases extended, division in media consisting of commercially available components.