Selective Viral and Rickettsial Serum Antibody Absorption by a Chromatographic Column

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Science  03 Feb 1961:
Vol. 133, Issue 3449, pp. 331-332
DOI: 10.1126/science.133.3449.331


Serum antibodies behave as cations at neutral pH and thus have low affinity for cellulose anion-exchange columns. Antigens of small size derived from adenovirus, influenza virus, and typhus rickettsiae, however, readily adsorbed to such columns. These adsorbed antigens specifically removed antibodies from antisera. This simple method permits antibody absorption by antigens ordinarily sedimented with difficulty.