Uptake of Tritium-Labeled Norepinephrine in Brain and Other Tissues of Cat in vitro

Science  07 Apr 1961:
Vol. 133, Issue 3458, pp. 1072-1073
DOI: 10.1126/science.133.3458.1072


Slices of cat cerebral cortex, heart, and spleen that have been incubated in media containing approximately 5 to 25 mµg of dl-norepinephrine-7H8 per milliliter contain levels of isotopic amine greater than those in the medium. The effects of norepinephrine concentration, reserpine, and ouabain on the uptake suggest that the amine enters cells both by diffusion and by a concentrating miechanism that is saturated at low levels of norepinephrine. The drugs inhibit the latter.