Germination of Bacterial Endospores with Calcium and Dipicoliniic Acid

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Science  26 May 1961:
Vol. 133, Issue 3465, pp. 1703-1704
DOI: 10.1126/science.133.3465.1703


Aerobic and anaerobic bacterial endospores can be germinated if calcium chloride and dipicolinic acid are added to well-washed suspensions. Maximum germination is obtained when the calcium and acid are present in a molar ratio of one or more. This suggests that the 1:1 chelate of calcium and dipicolinic acid is the agent that induces germination.