Contribution of E-Amino Groups to Ninhydrin Color Production in Proteins

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Science  09 Feb 1962:
Vol. 135, Issue 3502, pp. 441-442
DOI: 10.1126/science.135.3502.441


The ninhydrin color given by model compounds and by characterized proteins shows a consistent contribution from the ε-amino group of lysine of about 67 percent of that of an α-amino group, except in free lysine or in N-terminal lysine, where the ε-amino group makes a small contribution (7 to 10 percent) to the total color. This information can be applied to structure determination of Nε peptides of lysine.