Eliminative Split of Pectic Substances by Phytopathogenic Soft-Rot Bacteria

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Science  16 Mar 1962:
Vol. 135, Issue 3507, pp. 920-921
DOI: 10.1126/science.135.3507.920


Culture filtrates of Erwinia carotovora and Bacillus polymyxa split pectic substances to yield a product that absorbs at 230 mµ, and which reacts with thiobarbituric acid to form a substance that absorbs at 547 mµ. This product is believed to be a C-4,5-unsaturated oli-gouronide. The preferred substrate is polygalacturonic acid rather than pectin; the enzyme, provisionally named polygalacturonic-trans-eliminase, is activated by calcium.