An Artifact in Plant Autoradiography

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Science  03 Aug 1962:
Vol. 137, Issue 3527, pp. 343-344
DOI: 10.1126/science.137.3527.343


An artifact in autoradiography of bean plants containing phosphorus-32 is reported. It was shown that the apparent accumulation of isotope in primary leaves that were oven-dried prior to exposure to x-ray films was not metabolic but due to a drying gradient occuring within the leaf. This artifact disappears when autoradiographs are made of leaves that have been freeze-dried under vacuum.