Convenient Assay of Xanthine Dehydrogenase in Single Drosophila melanogaster

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Science  21 Sep 1962:
Vol. 137, Issue 3534, pp. 990-991
DOI: 10.1126/science.137.3534.990


Each fruit fly is homogenized in buffer and treated with charcoal. The enzyme is subsequently assayed by following the conversion of 2-amino-4-hydroxyptridine to isoxanthopterin in a sensitive fluorometer.