Defect in Small Millipore Filters Disclosed by New Technique for Isolating Oral Treponemes

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Science  12 Oct 1962:
Vol. 138, Issue 3537, pp. 139-140
DOI: 10.1126/science.138.3537.139


Three Millipore filters (100, 50, and 10 mµ) can effectively and easily be used to isolate oral spirochetes from a mixed oral inoculum. The spirochetes appear pure in the underlying agar, owing to their ability to grow through the filter. Their passage through the 10-mµ filters may be attributed to the instability of this pore size on storage.