Inter-tissue Complementation: A Simple Technique for Direct Analysis of Gene-Action Sequence

Science  12 Oct 1962:
Vol. 138, Issue 3537, pp. 149-150
DOI: 10.1126/science.138.3537.149


Sequence of gene action can be determined from unidirectional complementary interactions between synthetically active but genetically blocked tissues. A linear sequence of gene action has been constructed for anthocyanin synthesis in maize by this approach. This technique probably can be applied to other systems and organisms that have the requisite circumstances.