Two-Dimensional Gel Electrophoresis

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Science  12 Oct 1962:
Vol. 138, Issue 3537, pp. 152-153
DOI: 10.1126/science.138.3537.152


Improved resolution of serum protein mixtures is effected by electrophoresis, first in a 5 percent acrylamide gel following which a strip of the resolved pattern is embedded in 8 percent gel and subjected to a second electrophoresis separation at right angles to the first. Lactic dehydrogenase enzymes appear as small rectangular spots lying on an oblique straight line passing through the point of initial application.