Localizing Tritiated Norepinephrine in Sympathetic Axons by Electron Microscopic Autoradiography

Science  19 Oct 1962:
Vol. 138, Issue 3538, pp. 440-442
DOI: 10.1126/science.138.3538.440


Following intravenous infusion of tritiated norepinephrine, rat pineals were prepared for combined autoradiography and electron microscopy. Concentrations of photographic grains were observed only over regions of preterminal autonomic axons containing granulated vesicles, thereby directly demonstrating uptake of norepinephrine into these axons and strongly suggesting that their granulated vesicles contain norepinephrine.