Homology Test between the Nucleic Acid of an RNA Virus and the DNA in the Host Cell

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Science  14 Dec 1962:
Vol. 138, Issue 3546, pp. 1270-1272
DOI: 10.1126/science.138.3546.1270


Deoxyribonucleic acid in the host cell does not contain a sequence complementary to the nucleic acid of an RNA virus. Specific formation of hybridbetween deoxyribonucleic acid and ribonucleic acid was used as a detecting device. The test was internally controlled and sensitive enough to reveal complementary stretches in the DNA corresponding to 10 percent of the viral ribonucleic acid. The implications of the results for replication and transcription of RNA are discussed.