Chlorophyll a Appearance in the Dark in Higher Plants: Analytical Notes

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Science  05 Apr 1963:
Vol. 140, Issue 3562, pp. 49-51
DOI: 10.1126/science.140.3562.49


The increase in the ratio [chlorophyll a]/[chlorophyll b] which occurs in expanding bean leaves in the dark, is a result of chlorophyll a formation with a concomitant loss in chlorophyll b. The analytical methods for assay of these pigments were examined closely and found to be adequate for this purpose when proper safeguards are taken.