In vitro Culture of the Flagellate Protozoan Hexamita salmonis

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Science  19 Apr 1963:
Vol. 140, Issue 3564, pp. 290-292
DOI: 10.1126/science.140.3564.290


Trophozoites of Hexamita salmonis, asserted pathogen of juvenile salmonid fishes, were isolated from two species of Pacific salmon hosts and cultured repeatedly in an organic medium saturated with nitrogen. Primary isolates and serial subcultures usually exhibited five- to tenfold population increases per passage.