Albumin Replacement by Fatty Acids in Clonal Growth of Mammalian Cells

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Science  17 May 1963:
Vol. 140, Issue 3568, pp. 802-803
DOI: 10.1126/science.140.3568.802


Clonal growth of Chinese hamster strain CHD-3 cells in a synthetic nutrient mixture supplemented with 10 micrograms of purified fetuin per milliliter exhibits an apparent requirement for serum albumin. The albumin can be replaced by linoleic acid, which occurs as a tightly bound component of most albumin preparations. Linolenic acid and corn oil can also replace albumin, while oleic acid and esters of linoleic and linolenic acids cannot.