Reports

Chromosomal and Nucleolar RNA Synthesis in Root Tips during Mitosis

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Science  14 Jun 1963:
Vol. 140, Issue 3572, pp. 1231-1233
DOI: 10.1126/science.140.3572.1231

Abstract

Comparative rates of RNA synthesis in chromatin and nucleolar fractions during mitosis in root-tip cells of Allium and Nigella were studied by pulse-labeling of cells with tritiated cytidine. Although the rate of RNA synthesis decreases in the condensing chromosomes during prophase, it remains normal in the nucleolar fraction as long as nucleoli are maintained. RNA synthesis stops in mitotic cells lacking distinct nucleoli. In the late telophase or very early interphase cells, RNA synthesis resumes at a faster rate in the pronucleolar bodies than in the chromatin.