Microdetermination of Calcium by Aequorin Luminescence

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Science  21 Jun 1963:
Vol. 140, Issue 3573, pp. 1339-1340
DOI: 10.1126/science.140.3573.1339


A bioluminescent protein, aequorin, isolated from the jellyfish Aequorea in dilute disodium ethylenediaminetetraacetate solution, emits light on addition specifically of Ca++ or Sr++, thus providing the basis for a simple, quantitative micromethod for the determination of these cations, especially in biological fluids.