Calcium-Activated Adenosine Triphosphatase Localization in Cultured Beating Heart Cells

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Science  05 Jul 1963:
Vol. 141, Issue 3575, pp. 47-48
DOI: 10.1126/science.141.3575.47


The calcium-activated adenosine triphosphatase activity of cultured single beating heart cells of the rat was localized and visualized by incubating the cells, after extraction in a mixture of glycerol and water, with adenosine triphosphate and calcium. The calcium phosphate, precipitated at the site of the enzyme reaction, was converted into a visible precipitate. The enzyme activity appeared in bands perpendicular to the myofibrils.