Electron Microscope Autoradiography of Bacteria Labeled with Iodine-125

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Science  26 Jul 1963:
Vol. 141, Issue 3578, pp. 355-356
DOI: 10.1126/science.141.3578.355


The low-energy extranuclear electrons emitted by iodine-125 can be used for electron microscope autoradiography with results comparable to those obtained with tritium. Autoradiographs of tritium-labeled bacteria showed that 71 percent of all reacted grains localized on the cell. This figure was 86 percent when I125-labeled bacteria were used.