Malate Dehydrogenase: Multiple Forms in Separated Blastomeres of Sea Urchin Embryos

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Science  18 Oct 1963:
Vol. 142, Issue 3590, pp. 389-390
DOI: 10.1126/science.142.3590.389


Sea urchin embryos at the 64-cell stage were dissociated by treatment with trypsin and separated by centrifugation on a sucrose gradient. The large blastomeres have two and the small blastomeres have three bands of L-malate dehydrogenase activity, which are separated by disk microelectrophoresis on polyacrylamide gel, whereas unfertilized eggs have five.