Continuous Recording of Cell Number in Logarithmic and Synchronized Cultures

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Science  29 Nov 1963:
Vol. 142, Issue 3596, pp. 1183-1184
DOI: 10.1126/science.142.3596.1183


An instrument for the continuous recording of cell number has been developed and is being used to record changes in populations of logarithmic and synchronized cultures of protozoan flagellates. A Coulter cell counter is used in conjunction with a counting chamber that is fitted with a flexible polyethylene aperture (75 µ in diameter). This aperture rarely becomes blocked and appears to be self-clearing. The unit consists of a proportioning pump, a cell counter, a counting chamber, a rate meter, and a recorder.