Thymine Dimers and Inhibition of DNA Synthesis by Ultraviolet Irradiation of Cells

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Science  13 Dec 1963:
Vol. 142, Issue 3598, pp. 1464-1466
DOI: 10.1126/science.142.3598.1464


Measurements of the inhibition of DNA synthesis in bacteria and the formation of thymine dimers by ultraviolet irradiation of the cells indicate that one thymine dimer per 350-IA strand of DNA acts as a block to further synthesis. In a sensitive strain of Escherichia coli the blocks are permanent. In a resistant strain the blocks are only temporary but recovery of synthesis is not the result of splitting dimers.