Ethylene Oxide Sterilization of Tissue Culture Media

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Science  27 Dec 1963:
Vol. 142, Issue 3600, pp. 1654-1655
DOI: 10.1126/science.142.3600.1654


Liquid ethylene oxide, which kills bacteria, yeasts, and molds, was added to a tissue culture medium instead of the usual antibiotics. With some limitations, the use of ethylene oxide sterilization of tissue culture medium has been shown feasible. Data indicate that this method can be used in experiments involving the sterilization of test chemicals and components of the medium used for mammalian cells in culture.