Culture of Small Barley Embryos on Defined Media

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Science  27 Dec 1963:
Vol. 142, Issue 3600, pp. 1655-1656
DOI: 10.1126/science.142.3600.1655


Barley embryos, measuring 90 Fz in length, grew and became differentiated on a phosphate-enriched White's medium which was fortified with glutamine and alanine as major sources of organic nitrogen, and lesser amounts of five other amino acids. The pH of the medium was a critical factor in differentiation. On this medium, excised barley embryos developed more rapidly than embryos remaining in vivo, or embryos grown on media containing coconut milk.