Antiserums Prepared with Acrylamide Gel Used as Adjuvant

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Science  27 Dec 1963:
Vol. 142, Issue 3600, pp. 1677-1678
DOI: 10.1126/science.142.3600.1677


Antiserums to macroglobulins were prepared by a convenient and time-saving procedure in which acrylamide gel was used as adjuvant. After electrophoresis in acrylamide gel, the macroglobulin zone from the gel pattern was homogenized and injected directly into rabbits without intermediate isolation of the purified antigen. The resulting antiserums exhibited high titers of antiserum to macroglobulin and showed only weak cross-reactions with other gamma globulins in serologic tests.