Cytochrome Content of Mitochondria Stripped of Inner Membrane Structure

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Science  10 Jan 1964:
Vol. 143, Issue 3602, pp. 136-139
DOI: 10.1126/science.143.3602.136


The cytochrome composition of mitochondrial fractions which have been stripped of inner membrane subunits by exposure to high-frequency sound have been examined by lowtemperature spectroscopy. The ratio of cytochrome c to cytochrome a is not changed by the treatment, but the concentration of cytochrome per milligram of protein is increased and the concentrations of cytochromes c1 and b change slightly. These cytochromes (c1 and b) may be at least partly located in the subunits of the inner membrane, but the idea that all the respiratory components are located in single subunits of the mitochondrial cristae may be considered to be disproved.