Hybridization of Half Molecules of Rabbit Gamma Globulin

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Science  24 Jan 1964:
Vol. 143, Issue 3604, pp. 376-378
DOI: 10.1126/science.143.3604.376


Specifically purified rabbit antiovalbumin and normal γ-globulin labeled with 1-131 were dissociated into half molecules by reduction and acidification. When a mixture of the two preparations was neutralized, a large proportion of mixed molecules having active combining sites and the same sedimentation coefficient as the original γ-globulins was formed. Since the sulfhydryl groups were inactivated after reduction, the recombined subunits appear to be linked by noncovalent bonds.