Actinomycin D as a Probe for Nucleic Acid Secondary Structure

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Science  14 Feb 1964:
Vol. 143, Issue 3607, pp. 682-684
DOI: 10.1126/science.143.3607.682


The binding of actinomycin D to DNA results in an increase in the temperature at which thermal dissociation of the DNA occurs. With this enhancement of thermal stability as a criterion for binding, it is shown that complementary RNA and DNA-RNA hybrid do not bind actinomycin D. This result can be interpreted to mean that these polynucleotides have helical conformations which differ from that of DNA.