Electrophoretic and Immunological Studies of Squid Axoplasm Proteins

Science  06 Mar 1964:
Vol. 143, Issue 3610, pp. 1036-1037
DOI: 10.1126/science.143.3610.1036


By disc electrophoresis of the axoplasm of Dosidicus gigas, 14 protein bands have been resolved. Anti-bodies to the intra-axonal proteins and to squid blood proteins were produced in rabbits. By Ouchterlony's technique, six antigenic components can be demonstrated in axoplasm; the combined use of disc electrophoresis and immune diflusion in agar resolves seven antigenic components in axoplasm; none of these components is detectable in squid blood.