Reports

Freezing and Viability of Tetrahymena pyriformis in Dimethylsulfoxide

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Science  03 Apr 1964:
Vol. 144, Issue 3614, pp. 64-65
DOI: 10.1126/science.144.3614.64

Abstract

Ciliated protozoa may be preserved at very low temperatures. Tetrahymena pyriformis, suspended in 10 percent dimethylsulfoxide, survived when frozen in two steps, first to -20 ° C, then transferring immediately to a temperature of -196 ° C. Motility and ability to reproduce were recovered after freezing by this method and subsequent storage for 3 months in a liquid nitrogen refrigerator.

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