Reevaluation of Assays Used To Show RNA Induction of Glucose-6-Phosphatase in Ascites Cells

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Science  15 May 1964:
Vol. 144, Issue 3620, pp. 854-856
DOI: 10.1126/science.144.3620.854


The standard The standard procedures Glucostat method and Fiske-Subbarowphosphorus determination-are inadequate for the assay of glucose-6-phosphatase activity in Ehrlich ascites cells. Therefore, RNA induction of this enzyme in ascites cells, even if it occurs, cannot be demonstrated by thefe procedures.