Reports

Polarographic Investigation of Conjugated Fat-Soluble Vitamins

Science  29 May 1964:
Vol. 144, Issue 3622, pp. 1130-1131
DOI: 10.1126/science.144.3622.1130

Abstract

The half-wave potentials of all-trans β-carotene, all-trans retinol, 13-cis retinol, all-trans retinyl acetate, all-trans retinal, and vitamins D2 and D3 were related to the number of double bonds in conjugation. A minimum of three double bonds in conjugation was required before reduction took place at the dropping-mercury electrode. As the number of conjugated bonds increased in the fat-soluble vitamins, the initial reduction took place at a lower half-wave potential. All of the waves were linearly proportional to the concentration of the vitamins in the concentration range studied.

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