Serotonin Binding to Nerve-Ending Particles of the Rat Brain and Its Inhibition by Lysergic Acid Diethylamide

Science  29 May 1964:
Vol. 144, Issue 3622, pp. 1135-1137
DOI: 10.1126/science.144.3622.1135


The binding of serotonin to nerve-ending particles and other preparations from rat brain has been examined. By investigating the amount bound as a function of serotonin concentration from 10-7M to 1O-2M, it was possible to identify three major components having Kassoc (association constant) values of 2 x 106, 5 x 104, and 5 x 102. The component having the highest binding constant was not present in liver and appeared to be confined to the cortex and midbrain regions. This component is inhibited by d-lysergic acid diethylamide at low concentrations. Solubilization of this binding component has been achieved.